Tretinoin nrf2 reporter gene assay

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August undefined, 2024

WebDec 4, 2007 · Using a human mammary MCF7-derived AREc32 reporter cell line, we now report that all-trans retinoic acid (ATRA), and other retinoic acid receptor alpha … WebThe Assay System INDIGO's Human Nrf2 Reporter Cells include the luciferase reporter gene functionally linked to a promoter containing tandem antioxidant response elements (AREs). Thus, quantifying changes in luciferase expression in the treated reporter cells provides a sensitive surrogate measure of the changes in Nrf2 activity.

Pre-validation of a reporter gene assay for oxidative stress for the ...

WebMar 24, 2024 · Nrf2 is an essential transcription factor regulating the expression of genes containing an electrophile-responsive element (EpRE) responsible for the induction of a wide range of protective enzymes involved in the synthesis of GSH and protection of cells against electrophilic as well as oxidative stress [15,20].Due to the reactive electrophilic activity of … WebJournal: Scientific Reports Article Title: Effect-based assessment of recipient waters impacted by on-site, small scale, and large scale waste water treatment facilities – combining passive sampling with in vitro bioassays and chemical analysis doi: 10.1038/s41598-018-35533-x Figure Lengend Snippet: Derived curve fit parameters for … hartley investment trust limited

Cignal Reporter Assay Handbook - Qiagen

WebJan 15, 2014 · To facilitate interpretation of the data and to avoid artefacts, all three reporter-gene assays used simple and defined reporter genes and a similar cellular basis, … WebMar 24, 2024 · It was shown that these three α-dicarbonyl compounds react instantaneously with GSH, with the GSH-mediated scavenging decreasing in the order MGO > GO > 3DG. Furthermore, in a cell-based reporter gene assay MGO, GO, and 3-DG were able to induce Nrf2-mediated gene expression in a dose-dependent manner. WebAug 18, 2024 · Results show that PG was a more potent inducer of Nrf2-mediated gene expression than EGCG, with a 5% benchmark dose (BMD5) of 0.35 µM as compared to 2.45 µM for EGCG in the reporter gene assay. EGC and GA were unable to induce Nrf2-mediated gene expression up to the highest concentration tested (75 µM). hartley iowa cemetery

In vitro–In vivo Hybrid Approach for Studying Modulation …

Tretinoin nrf2 reporter gene assay

Luciferase Reporter Assay Kits For Cell-Based Screening INDIGO

WebFor the NRF2 reporter gene experiments, the results were presented as the Induction Factor (IF), which is the measured relative light unit (RLU) value divided by the mean RLU value of the solvent control. When the induction factor of curcumin was over 8, the NRF2-U2OS reporter gene assay was regarded to be effective. WebMay 23, 2024 · As a step towards this direction, we previously developed a transient reporter gene assay for analysis of oxidative stress in D. rerio fibroblast (ZF4) and liver (ZFL) cell lines (Lungu-Mitea et al. 2024), by measuring induction of the nuclear factor erythroid 2-related factor 2 (Nrf2), a major upstream regulator of ROS detoxification and …

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WebApr 11, 2024 · Download Citation Engineered Microencapsulated Lactoferrin Nanoconjugates for Oral Targeted Treatment of Colon Cancer Despite current progress in the development of targeted therapies for ... WebDec 15, 2008 · Abstract. Clinical use of retinoic acids (RA) is hindered by toxicity possibly related to oxidative stress. Recently, RA at relatively low concentrations was shown to …

WebWe found that mutations at K525, K535, and K591 resulted in impaired Nrf2-dependent gene transcription, as measured by the luciferase reporter gene assay. To understand how sumoylation influenced transcription we assessed, using the Re-ChIP (Active Motif, Carlsbad,CA) assay, whether sumoylation impacted the ability of Nrf2 to interact with its … WebJun 1, 2024 · Recently, Mertl et al. (2024) reported the application of the dual-luciferase system for the ARE-Nrf2-mediated reporter gene assay, NanoLuc, to determine the ARE-Nrf2-mediated transcriptional activity, and of the Firefly luciferase assay for evaluation of the cytotoxicity, to detect the presence of sensitizing chemicals in medical devices.

WebEach Cignal Reporter Assay Kit is specific for a particular transcription factor and/or signal transduction pathway, and includes 3 components. The first is a transcription factor-responsive firefly luciferase reporter, which encodes the luciferase reporter gene under the control of a basal promoter element, the TATA WebMay 7, 2013 · The reporter assay demonstrated that Nrf2 induced the promoter activity of Mrp1 gene after co-transfection of pGL3-wt with Nrf2 expressing plasmid (Figure 4, panel …

WebARE-GFP报告基因是翌圣生物自主研发的用于检测ARE转录活性水平为目的的报告基因。ARE(antioxidant response element) 与Nrf2和Nrf1转录因子结合，调节参与保护细胞免受氧化损伤的相关基因。 ARE-GFP报告基因主要应用于检测细胞Antioxidant Response信号通路、药物研究、基因过表达和RNAi的表型分析等。

WebSep 28, 2024 · Nuclear factor erythroid-2 related factor 2 (NRF2) encoded by the NFE2L2 gene is a transcription factor critical for protecting cells from chemically-induced oxidative stress. We developed computational procedures to identify chemical modulators of NRF2 in a large database of human microarray data. A gene expression biomarker was built from … hartley iowa dog trainerWebSignosis Inc nrf2 are luciferase reporter hek 293 stable cell line. Nrf2 Are Luciferase Reporter Hek 293 Stable Cell Line, supplied by Signosis Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more. hartley iowa assisted livingWebTransient transfection of HepG2 cells with a reporter plasmid of the upstream region of the human PST gene caused a significant increase in reporter gene activity after gallic acid exposure. Moreover, gallic acid increased the nuclear levels of Nrf2, a transcription factor governing antioxidant response element (ARE). hartley iowa chamber of commerceWebcysteine residue, resulting in its dissociation from the transcription factor Nrf2 (nuclear factor-erythroid 2-related factor 2). The dissociated Nrf2 can then activate ARE-dependent … hartley iowa countyWebthe main components and procedures of the keratinocyte-based ARE-Nrf2 luciferase reporter gene test method. Preparation of the Keratinocyte Cultures 16. Transgenic cell … hartley iowa funeral home obituariesWebAug 6, 2013 · In the present study, Nrf2 activators were identified using cell-based luciferase enzyme fragment complementation (EFC) assay, and the mechanism of Nrf2 activation … hartley iowa city hallWebMar 25, 2024 · Nrf2 Transcription Factor Assay Kit (Colorimetric) (ab207223) is a high throughput assay to quantify NF-E2-related factor (Nrf2) activation. This assay combines … hartley iowa map