WebDec 20, 2024 · Atlantis HILIC ANSWER Equilibrate in 50% acetonitrile/50% aqueous buffer (10 mM final buffer concentration) for 50 column volumes. Prior to the first injection, equilibrate with 20 column volumes of initial mobile phase. When running gradients, equilibrate with 10 column volumes between injections. ADDITIONAL INFORMATION WebA recom- mended starting buffer concentration is 5 to 10 mM and increase up to 20 mM for improved peak shape and reten- tion. A typical pH range for HILIC separations will be pH 2 …
Effect of acetonitrile content on retention trends in HILIC and …
WebMar 1, 2009 · HILICON AB Abstract and Figures We provide information and advice to our customers on application technologies and regulatory matters to the best of our … WebThe maximum recommended sample volume is 1.5 ml. See Figure 2 for the effect of varying the sample volume applied to the column using a syringe. Use a flow rate between 1 and10 ml/min. Discard the eluted buffer from the column. 3 Change to buffer and proceed with injection. If the sample volume is less than 1.5 ml, add buffer until a total of im right here waiting for you
Analysis of cephalosporins by hydrophilic interaction ... - PubMed
WebIn addition, it is critical to optimize pH and buffer strength for the best results. A recommended starting buffer concentration is 5 to 10 mM and increase up to 20 mM for improved peak shape and retention. A typical pH range for HILIC separations will be pH 2 to 7 using formate and acetate buffers. Applications WebHILIC Troubleshooting ‹ This section is not an exhaustive guide to HPLC column and instrument troubleshooting but an aid to help you understand and correct some of the more common chromatographic problems that … WebMar 28, 2016 · Storage of HILIC columns with buffer is recommended to decrease equilibration time. Storing a column with buffer in the mobile phase is typically not recommended for a number of reasons, most have little to do with sorbent stability. Buffers can precipitate if the organic concentration is too high but water will evaporate first. imright insurance