H69 cholangiocyte cell line

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August undefined, 2024

WebMay 1, 2024 · Cell lines. The cholangiocyte cell line H69 is a SV40-transformed bile duct epithelial cell line derived from a non-cancerous human liver [23]. H69 cells were … WebNational Center for Biotechnology Information

The leukocyte activation receptor CD69 controls T cell

WebJun 1, 2004 · The cholangiocyte cell line H69 is a SV40-transformed bile duct epithelial cell line derived from a non-cancerous human liver [53] and was obtained in 2010 from Dr. Gregory J. Gores, Mayo Clinic ... WebCell Culture. H69 cells, immortalized human cholangiocytes, were obtained from Dr. Doug Jefferson (Tufts University, Boston, MA) and cultured as previously described.16 For … janus henderson forty fund prospectus

H69 91091802 Sigma-Aldrich

WebCholangiocyte cell lines and primary cholangiocytes were used for in vitro studies. ... were obtained as previously described. 15–17 The H69 human cholangiocyte cell line was provided by Dr. Nicholas LaRusso (Mayo Clinic, Rochester, USA). Cells were grown in DMEM/F12 supplemented with 10% fetal bovine serum, 1% penicillin/streptomycin ... WebWe used the human cholangiocyte cell line (H69) and assessed it for expression and function of an a... View. Cathepsins as effector proteases in hepatocyte apoptosis. Article. Feb 1999; WebH69AR CRL-11351 ™ H69AR is an epithelial cell isolated from the lung of a 55-year-old White male patient with carcinoma. This cell line was deposited by S. Cole and can be used for cancer research. Product category Human cells Organism Homo sapiens, human Cell type epithelial cell Morphology epithelial Tissue Lung Disease Carcinoma Applications janus henderson fund factsheets

Human Cholangiocyte Primary Cell Culture - Frozen Vial

Generation of functional cholangiocyte‐like cells from human

WebOct 12, 2024 · Here, exosome-mediated transfer of mRNAs from the human cholangiocyte cell line (H69) was investigated following exposure to Ov- GRN-1, to naïve recipient H69 cells. To minimize the influence of endogenous huPGRN, the gene encoding huPGRN was inactivated using CRISPR/Cas9-based gene knock-out. WebCell Line Description. This cell line has been established from the pleural fluid of a 55 year old Caucasian male with small cell carcinoma of the lung prior to treatment. Inoculated … janus henderson funds 2022 distributionsWebAug 26, 2015 · Protein levels for p15, p21, and CCNE1in the normal cholangiocyte cell line H69 also decreased, although p-Rb levels seemed unaffected. The impact of targeting miR-224 in CCA on normal cholangiocytes remains to be investigated as p-Rb levels did not change upon miR-224 stimulus, but an effect of further downregulation of miR-224 … janus henderson forty fund d shares

"WebApr 13, 2015 · Furthermore, the number (iDC: 7941, H69: 9724, NHC: 9295) and magnitude (Supplementary Figure 4) of differentially expressed genes are ultimately similar to both cholangiocyte cell lines. " - H69 cholangiocyte cell line

H69 cholangiocyte cell line

Development and characterization of cholangioids from normal …

WebCholangiocyte cell line. Cholangiocytes are epithelial cells which line the bile ducts. Please note that there is a distinct lung carcinoma cell line with the same name (H69). … Webtumor-derived cholangiocyte cell lines. Table 1 presents an overview of cell sources used for bile duct tissue engi-neering, discussed in the next paragraphs. ... biliary epithelial cell line H69.40 H69 cells presented key biliary markers and were used to study anion exchanger (AE) activity41 and epidermal growth factor (EGF) receptor

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WebThis cell line is aneuploid, will form colonies in soft agar and retains small cell carcinoma morphology and ultrastructure as well as APUD cell characteristics. The cells grow in aggregates, thus cell counts are not accurate. The cells stain positively for cytokeratins. … WebThe human cholangiocytes (H69), provided by Dr. Douglas Jefferson (New England Medical Center, Tufts University, Boston, MA), were cultured as described. Infection of Cells and Inhibitors. RRV infection of cell lines was performed in 24‐well culture plates containing cells seeded at 4 × 10 5 /well and incubated at 37°C for 3 days. Confluent ...

WebMay 15, 1999 · In summary, the human cholangiocyte cell line H69 possesses a functional bile acid transporter, and GUDC decreases BV-induced apoptosis and inhibits activity of caspase 3 protease by blocking CC release from mitochondria.

WebApr 28, 2024 · human cholangiocyte cell line, H69, a CCA cell line CC-LP-1, CagA + ve Helicobacter pylori, 40. and H. bilis bacilli. Exposure to increasing numbers of H. pylori at 0, 1, 10,100 bacteria per . 41. WebDec 13, 2024 · H69 cells, an SV40-transformed (i.e., immortalized) human cholangiocyte cell line , were grown in DMEM Nutrient Mixture F-12 (DMEM/F-12) + GlutaMax supplemented with 10% FBS and 100 U/mL penicillin-streptomycin. RAW264.7 mouse macrophages were from the American Type Culture Collection (ATCC) and were …

WebProduct Description. ". Human Cholangiocyte Primary Cell Culture - Frozen Vial. Source: Human Biliary Epithelial Cells. Positive Markers: CK7, CK19, glutamyl transpeptidase, …

WebJul 24, 2024 · The human cholangiocyte H69 cell line is a SV40-transformed human bile duct epithelial cell line derived from a normal liver 61. The H69 cells were used between passages 10 and 30 and grown in H69 medium that included 43.82% DMEM medium (Dulbecco’s Modified Eagle’s Medium), 43.82% DMEM/Ham’s F12, 10% fetal bovine … lowest worm god floatWebSep 11, 2024 · The normal human cholangiocyte cell line (NHC) was isolated from a normal human liver and characterized by Joplin et al 17 and was a gift from Dr Juan Medina (University of Navarra, Pamplona ... janus henderson financial advisorsWebFor construction of the H69 cholangiocyte cell line stably expressing miR-506, we used the BLOCK-IT PolII miR RNA Expression Vector Kit with EmGFP (Invitrogen). Pre-miR … janus henderson global equity accumulationWebPrimary sclerosing cholangitis (PSC) is a chronic, idiopathic cholangiopathy. The role of cholangiocytes (biliary epithelial cells) in PSC pathogenesis is unknown and remains an … janus henderson forty priceWebWe have also shown an increase in the p27 cyclin dependent kinase inhibitor due to inhibition of proteasomal regulation by TGFβ in human H69 cholangiocytes. 24 In the human cholangiocarcinoma cell line HuCCT1, … janus henderson forty nWebFigure Legend Snippet: Expression of GIRK1 in some SCLC cell lines but not in normal SAE cells, nor in adenocarcinoma Clara cell phenotype cell lines . Top panel: GIRK1 is … lowest work week countryWebDec 5, 2005 · Cell lines. The human mutant cell line T2 (174 × CEM.T2) 22 and the human CML cell line K562 transfected with full-length cDNA encoding the HLA-A * 0201 allele, … lowest worth currency 2019