Crystallin promoter

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August undefined, 2024

WebRegulation of Mouse Small Heat Shock Protein alpha b-Crystallin Gene by Aryl Hydrocarbon Receptor. Author: Liu, S. A. L. S. A. Piatigorsky, J. Author Address WebBy use of a gene fusion of the alpha A-crystallin promoter to the viral immortalizing oncogene, polyoma large T antigen (PyLT), we created transgenic mice that express PyLT specifically in ocular lens. Expression of large T antigen during embryonic development led to a perturbation in lens development, specifically, an interference with the ...

Gamma Crystallin - an overview ScienceDirect Topics

WebCrystallins are a diverse group of abundant soluble proteins that are responsible for the refractive properties of the transparent eye lens. We showed previously that Pax-6 can activate the αB-crystallin/small heat shock protein promoter via the lens-specific regulatory regions LSR1 (−147/−118) and LSR2 (−78/−46). WebDec 3, 2013 · CpG island methylation in the αA-crystallin promoter was similar in age-related cataract and high-myopic cataract classified as Lens Opacity Classification System III Grade Nuclear Color 2–3. Pyrosequencing, a real-time DNA sequencing method, was used to determine single-base variations caused by CpG methylation of the CRYAA … birds eye frozen vegetables products

What is crystallin promoter? Technology Trends

WebIn anatomy, a crystallin is a water-soluble structural protein found in the lens and the cornea of the eye accounting for the transparency of the structure. It has also been … WebThe inclusion of a Pax6 consensus binding site within the -282/+43 alphaA-crystallin promoter enhances the ability of this promoter to drive transgene expression in the lens … WebThis present study is the first to show that mammalian α-crystallin promoter function can be analyzed in zebrafish embryos by observing green fluorescent protein (GFP) … birds eye garlic chicken recipe

What is crystallin promoter? Technology Trends

WebI am a highly motivated and passionate individual with a talent for organization and project management. I have enjoyed leading teams to complete projects in various industries … WebCrystallin promoters of widely different species are dependent on the same suite of transcription factors and function in similar ways in lens-cells of other species both in culture and in transgenic models (for example: [31–33]). This allows comparison of promoters from both chicken and mouse in mouse cells. birds eye gluten free fish fingersWebEarlier experiments showed that the betaB1-crystallin promoter sequence from -120 to -48 contains a cis element (PL2 at -90 to -76) that stimulates the activity of a heterologous promoter in lens cells but not in fibroblasts. birds eye golden crunch chips

"WebFeb 21, 2024 · The sequence between SacI and AfeI was replaced with a sequence containing CG-depleted chicken lens δ1-crystallin gene (Sasaki) promoter 51, a … " - Crystallin promoter

Crystallin promoter

Characterization of the chicken L‐Maf, MafB and c‐Maf in crystallin ...

WebDec 1, 2012 · The gene promoter contains response elements for some key transcription factors essential for normal lens development. Prominent among these are factors such as Pax6, c-Maf, Sox2, Prox1, and Six3 [93,94]. In many crystallin gene promoters, elements for lens and non-lens expression are combined in the same 5 ′ region. WebMar 30, 2024 · Stimulation of αA-crystallin promoter activity via 8-bromo-cAMP, forskolin, or human T-cell leukemia virus type I Tax1 in transfections and reduction of activity of this site in cell-free transcription tests by competition with the somatostatin CRE supported the idea that DE1 is a functional CRE. Finally, Pax-6, a member of the paired-box ...

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WebMar 1, 2003 · In order to study lens-retina relationships during development, we cloned the zebrafish alphaA-crystallin cDNA and its promoter region. Using a 2.8-kb fragment of the zebrafish alphaA-crystallin promoter (z (alpha)Acry), we expressed the diphtheria toxin A fragment (DTA) in zebrafish embryos in a lens-specific manner. Web300bp Human Beta B1 crystallin promoter. Availability. Academic Institutions and Nonprofits only Enlarge mEos2-Talin-N-22. Plasmid #57420. Purpose. Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581. Depositor. Michael Davidson. Article. Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi ...

WebMar 21, 2024 · Recruits the transcriptional coactivators CREBBP and/or EP300 to crystallin promoters leading to up-regulation of crystallin gene during lens fiber cell … WebJan 15, 2000 · Many of the crystallin promoters have been carefully dissected into component cis -acting elements with critical regions and putative transcription factor binding sites identified ( Cvekl and Piatigorsky, 1996; Duncan et al., 1996; Matsuo et al., 1991 ).

WebDec 29, 2008 · The scallop Ω-crystallin promoter sequence contains an abundance of motifs that are similar to those in vertebrate crystallin promoters. These include cis …

Web76 of this system. Data on crystallin promoter activity would also facilitate the expression 77 of introduced proteins in zebrafish lens and other tissues. 78 79 A comparison of mouse and zebrafish -crystallin promoter activity can also help detail 80 the evolution of tissue specific expression. Past studies have examined the evolution of

WebMar 1, 1989 · Recently Landel et al. (1988) have combined the α -crystallin promoter with the ricin cytotoxic gene to produce a single line of transgenic mice with a somewhat more severe phenotype consisting of a still smaller lens and other interesting eye malformations. dan and trish bellWebRetrospectively from the results of ( Maf) knock-out mice. it was also shown that MAF binds the promoter sequences of some, B-crystallin promoters ( Ring et al., 2000 ). Three … birds eye garlic chicken cooking instructionsWebApr 8, 2024 · Further explorations unveiled the increased MAF in high myopia and its participation in a direct activation of crystallin promoters and an indirect activation via … dan and trishWebThe J3-crystallin promoter (Kozmik etal. 2003) contains a pair of PaxB/Pax6-binding sites though its complete promoter sequence remains to be reported. Finally, two squid (Ommastrephes sloani) crystallin promoters have been characterized using vertebrate lens nuclear extracts (Tomarev etal. 1994). The SL11 and SL20 crystallin promoters … dan and tom minecraftWebSep 1, 2002 · These data suggest that the chicken β B1-crystallin promoter is the promoter of choice to direct the high level ectopic expression of RNAs to lens fiber cells … dan and tom weddingWebA DCR1/αA-crystallin promoter (including DCR2) coupled with EGFP virtually recapitulated the expression pattern of αA-crystallin in lens epithelium and fibers. In contrast, the DCR3/αA/EGFP reporter was expressed only in 'late' lens fibers. Chromatin immunoprecipitations showed binding of Pax6 to DCR1 and the αA-crystallin promoter … dan and trish willoughbyWebThe beta-B1 crystallin promoter produced GFP expression in the lenses of 37% of injected embryo’s after 48 hours. Fifty percent of MO injected … dan and wendy anniversary photo