Cck8 thp-1
WebJun 1, 2024 · After 5 days of culture, primary cortical neurons were stimulated with addition of varying concentrations of Aβ25-35 or CM obtained from THP-1 monocytes. 2.5. Detection of cell viability using cell counting kit-8 (CCK8) Cells for each treatment group were counted and seeded in 96-well plates at 100 μl/well in quintuplicate for each treatment … WebMar 20, 2024 · 1.3.8 Western blotting检测目的基因表达 取4组细胞(Control组、耐药组、 NC组以及FOXO4-OERNA组)分别用磷酸缓冲盐溶液洗涤，然后于浓缩胶和分离胶中分别电泳，在4℃、300 mA恒流条件下电转150 min，将蛋白转移到 PVDF膜上。 ... LncRNA H19在THP-1来源泡沫细胞脂质蓄积中的作用 ...
Cck8 thp-1
Did you know?
WebMar 26, 2024 · CCK8 results indicated that over-expressed lncRNA NR104098 can inhibit cell proliferation of NB4 and THP-1 cells in a time-dependent manner . To evaluate the effect of lncRNA NR-104098 on proliferation in AML cell lines, the expression of the proliferation-associated protein ki67 was determined by immunofluorescence staining analysis ( … Web7月17日收录于话题ZetaLifeAdvancedDNARNA，AD600150转染试剂；成功转染THP-1(人单核白血病细胞)悬浮细胞；来自广州中山大学附属**医院客户的喜讯;出色的细胞转染结果与您分享;24小时荧光图片:zetalife，AdvancedDNARNA转染试剂信息如下 ...
WebBioAssay record AID 1767130 submitted by ChEMBL: Antiproliferative activity against human THP-1 cells assessed as reduction in cell viability incubated for 72 hrs by CCK8 assay. WebTIB-202 ™. THP-1 is a monocyte isolated from peripheral blood from an acute monocytic leukemia patient. This cell line can be used in immune system disorder research, immunology research, and toxicology …
WebHuman monocyte cell line THP-1 cells were obtained from Procell Life Science & Technology (Wuhan, China). RPMI-1640 was a suitable medium for THP-1 cells and was obtained from Gibco. THP-1 cells in FBS concentration and CO 2 incubator conditions were the same as ATDC5 cells. PMA (Phorbol 12-myristate 13-acetate, 25 ng/mL, Meilunbio, … WebFeb 15, 2024 · To explore the importance of mTORC1 pathway in the synergistic anti-leukemia effect of Ara-C/metformin, MHY1485 (mTOR agonist) was added to treat HL-60 …
Web瑞禧生物提供科研级Metronidazole-PEG-Glucose 甲硝唑-聚乙二醇-葡萄糖,我们一直致力于研发纳米靶向科研产品以满足国内广大科研院校及其他相关科研单位的需求。Metronidazole-PEG-Glucose 甲硝唑-聚乙二醇-葡萄糖试剂不能用于人体治疗、药物开发、和其他商业用途。如果需要采购Metronidazole-PEG-Glucose 甲硝唑-聚 ...
WebJun 21, 2024 · 2.6 Cell counting kit-8 (CCK8) assay Transfected cells were adjusted to 3 × 10 4 /ml and plated into 96-well plates (3000 cells/well in 100 µL medium). After culturing for 24, 48, 72, and 96 h, the supernatant was replaced with 100 µL of serum-free medium added with 10 µL of CCK8 (Dojindo, Japan) and incubated at 37°C in the dark for 1 h. the advertiser njWebObjectives: To investigate the underlying mechanism of GAA inhibition of inflammation and lipid deposition in human monocyte (THP-1) cells. Material and methods: The Cell Counting Kit-8 (CCK-8) assay was used to assess the potential effect of GAA on the viability of THP-1 cells. The release of inflammatory cytokines and oxidative stress was ... the fried foundationWebSep 3, 2024 · We found that in THP-1-derived macrophages, LPS obviously increased SVIL mRNA and protein expression. Inhibition of TLR4 by Resatorvid (Res) remarkably reversed the LPS-induced SVIL expression. ... However, CCK8 data showed that SVIL knockdown did not affect the cell viability compared with control group with or without PMA incubation ... the advertiser north greenbushthe advertiser news spring hill tnhttp://muchong.com/t-8264924-1-pid-1 the advertiser newspaper rensselaer countyWebNational Center for Biotechnology Information the advertiser rensselaer county editionWebJun 1, 2024 · THP-1 cells were pre-treated with or without various concentrations of DHA in RPMI 1640 with 0 % FBS for 24 h, followed by 50 μM Aβ25-35 treatment for 3 d. CM … the advertiser rensselaer county